Monthly Archives: February 2014

STORM Images of Muscle Fibers

Below are the STORM images on muscle fibers we took few days ago, because of lower fluorescence intensity, the quality of the storm images are not very good, but we see some two band structures in the red channel, it more »

Posted in Jianquan Notes, Kner Lab Notes, Team Blog | 5 Comments

Minutes of the Feb. 19th Phone Meeting

This meeting began on time and included all PIs, post-doc Jianquan Xu, grad students Qirui Fan and Kayvan Tehrani, research assistant Nati Chen; and Karine Thate from MOS. Updates on Papers, Presentations and Meetings: – Jessica received comments and questions more »

Posted in Alpert Lab Notes, Team Blog | Comments Off on Minutes of the Feb. 19th Phone Meeting

Latest STORM Images of HEPG2 cells

Here are two of our recent STORM images, one stained with QD565 (30nM concentration of secondary Antibody), and the other stained with Alexa488 in presence of MEA buffer.

Posted in Kner Lab Notes, Team Blog | Comments Off on Latest STORM Images of HEPG2 cells

Muscle Fiber Staining – What we expect to see…

Hi Jianquan and the rest of the Kner lab, Just wanted to send you an diagram of what we would expect to see with the phalloidin/Alexa488 and tropomodulin/Alexa680 label. Phalloidin labels actin, which makes up the thin filaments, and tropomodulin more »

Posted in Brainerd Lab Notes, Team Blog | 3 Comments

Imaging Relaxed Rabbit Psoas Fibers

Hi Jianquan, Last week, I stained the relaxed muscle fibers with alpha-actinin/Alexa688. I have attached some images to this email. As you can see, we are still getting thick bright band, and almost equally thick dark bands. It definitely seems more »

Posted in Brainerd Lab Notes, Team Blog | Comments Off on Imaging Relaxed Rabbit Psoas Fibers

Ready to test 2-colour stained Rabbit Muscle Fibers

Hi Nati, We are ready to test the two color stained fibers, we can get started to test some samples next week if you can send us. Sorry, I just read your last message again, did you want me to more »

Posted in Jianquan Notes, Kner Lab Notes, Team Blog | 1 Comment

Working together to deal with QD-nonspecific binding in fluorescence imaging

Hello QSTORM team, As discussed in the previous QSTORM meeting, we all face the same QD-nonspecific binding issue. I’m working on the particle optimization, and meanwhile I was wondering if you can send me your QD cell staining protocols including more »

Posted in Team Blog, Winter Lab Notes | 2 Comments

Publication of Powerful new Super-Resolution Imaging Technique

Using a DNA-tagging technique instead of immunohistochemistry, researchers were able to produce 10 nm resolution STORM images labeling four distinct cellular structures -with four different colors – in a single fixed cell.  The technique was developed by Peng Yin’s lab more »

Posted in Alpert Lab Notes, Team Blog | Comments Off on Publication of Powerful new Super-Resolution Imaging Technique