Monthly Archives: December 2013

Quantum Dot Labeling

Hello. Kayvan and Jianquan have also made progress with quantum dot labeling. The image shows widefield (lower left) and STORM images of microtubules in HEPG2 cells labeled with 545nm QDs (commercial). The labeling is still not perfect, but it’s progress. more »

Posted in Kner Lab Notes, Team Blog | 1 Comment

Rabbit Psoas Muscle with MEA buffer

Hello, Kayvan and Jianquan have looked at the samples Nati sent. Below is a widefield image of a sample Kayvan prepared yesterday. For whatever reason, the labelling appears to be weak and sparse. Kayvan also tried a STORM image, the more »

Posted in Kner Lab Notes, Team Blog | 1 Comment

Muscle Cells Shipped

Jianquan – Sample was shipped today, for arrival tomorrow afternoon. — Beth

Posted in Brainerd Lab Notes, Team Blog | 3 Comments

Fire in the hole: QSTORM Paper Submitted

I have submitted the paper to Nanotechnology. You should receive an email confirmation. In the meantime, you can use this citation: 1. J. Xu, Q. Fan, K.D. Mahajan, G. Ruan, A. Herrington, K.F. Tehrani, P. Kner, J.O. Winter, Micelle-templated composite more »

Posted in Team Blog, Winter Lab Notes | 1 Comment

Test muscle cells in MEA buffer

Hi Dr. Brainerd, As we talked in the phone meeting yesterday, we are using the MEA buffer now, and it seems very helpful to improve the blinking of Alexa dyes. So we are thinking maybe we can test some muscle more »

Posted in Jianquan Notes, Kner Lab Notes, Team Blog | 4 Comments

Minutes of the Dec. 11 Phone Meeting

All PIs and post-doc Jianquan Xu participated on this final phone meeting of the year. While Carnegie-Mellon is in the midst of final exam week, Ge’s two new students are making progress mastering the process of culturing untagged Invitrogen quantum more »

Posted in Alpert Lab Notes, Team Blog | Comments Off on Minutes of the Dec. 11 Phone Meeting

Troubleshooting Commercial Quantum Dots

Due to our problems with the commercial quantum dots, I called customer support at Invitrogen. The staff suggested that I should fix and permeablize the fibers before I incubate them in the antibodies/qdots. So, I changed my protocol to include more »

Posted in Brainerd Lab Notes, Team Blog | 1 Comment

Testing Nonspecific Qdot binding

  Last week, Qirui sent us quantum dots that are not conjugated with IgG’s to test for nonspecific binding. Without an antibody conjugate, the quantum dots should wash off the muscle fiber. So ideally, we should see no fluorescence. However, more »

Posted in Brainerd Lab Notes, Team Blog | 2 Comments

QSTORM presentation at MRS Fall Meeting

Last week, I shared our “Making Molecular Movies with QSTORM” presentation at the 2013 Fall Meeting of the Materials Research Society at Hynes Convention Center in Boston. The presentation was part of the Public Outreach Center’s activities – it took more »

Posted in Alpert Lab Notes, Team Blog | Comments Off on QSTORM presentation at MRS Fall Meeting

Mailed more samples

Hi Nati, I mailed some new samples and you will probably get it tomorrow morning. There are two vials. The first vial labeled “COOH” is QD without IgG, and it serves as control; the other one has the label of more »

Posted in Team Blog, Winter Lab Notes | 4 Comments