Monthly Archives: September 2013

Minutes of the 9/4/13 Phone Conference

All hands were present on the 9/4 phone conference, including postdoc Jianquan, who had just settled into his new digs in Athens for his rotation in the Kner Lab at the University of Georgia.  Jianquan is working with tissue culture more »

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Brainerd Lab: Phalloidin/Alpha-actinin Double Stain & Troubleshooting Tmod/Commercial Qdots

Two weeks ago, I prepared a couple slides of rabbit psoas muscle fibers stained with both phalloidin and alpha-actinin IgG antibodies. Phalloidin is a toxin that stains actin, a protein that makes up muscle thin filaments. As you can see more »

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2 Color STORM imaging

Here is the result of our newest 2 color STORM imaging on Rabbit Psoas. Red channel is alpha-actinin and Blue channel is Phalloidin. Image 1 is widefield Z-stack of the sample, and images 2-4 are STORM images on the same more »

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Question from audience member on QD – Gold NP brightness

Jessica or Qirui – After my QSTORM stage presentation this afternoon, I had a question from an audience member that I wasn’t sure how to answer. He asked if the gold nanoparticle totally quenches the quantum dot at a certain more »

Posted in Alpert Lab Notes, Team Blog | 6 Comments

Captioned Video & NISE Net package for “Making Molecular Movies with QSTORM”

I’ve continued to present our QSTORM stage presentation, “Making Molecular Movies with QSTORM”, throughout the summer at the Museum of Science. Audience response continues to be overwhelmingly positive. To date, I’ve presented this program 53 times to audiences totaling just more »

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Hello, UGA; Hello, Bulldog

It has been already 3 weeks since I arrive at Athens, should update this earlier, but I am always a procrastinator. It is not a big city, smaller but much quieter than Pittsburgh. Everything is good here, except the hot more »

Posted in Jianquan Notes, Kner Lab Notes, Team Blog | 2 Comments

QD-IgG not working as expected

Dr. Brainerd, I heard that the QD-IgG were not working as expected. Could you give me some details about the experiments, so that I can understand the problem better and make improvements accordingly? Thanks! Qirui

Posted in Team Blog, Winter Lab Notes | 1 Comment

Muscle Images

Hi Beth, Here’s a new STORM image of the Rabbit muscle. Blue is the actin. Red is the alpha-actinin. The acquisition wasn’t very long so there aren’t that many localizations but I think the red channel looks more reasonable than more »

Posted in Kner Lab Notes, Team Blog | 2 Comments

Brainerd Lab QD controls

Because the signal from our tropomodulin/qdot labelled muscle fiber was diffuse and required high laser power to see, we decided to prepared four control slides. These slides included muscle fiber only, muscle fiber plus block only, muscle fiber plus primary more »

Posted in Brainerd Lab Notes, Team Blog | 1 Comment