Monthly Archives: September 2011

Immunostaining of PC12 Cells

Yiyi has showed us the immunostaining protocol for PC12 cells. Immunostaining is a process that uses antibodies to tag proteins of interest in cells and tissues. We used a mouse tau antibody as the primary antibody to attach on to more »

Posted in Team Blog, Yang Lab Notes | 1 Comment

Micelle vs. commercial QD oxidation times

This test was intended to show the difference in oxidation times between Dr. Ruan’s micelles and commercial quantum dots.  Both slides were made by mounting and then drying the dots on a coverslip and then using water as a media.  more »

Posted in Kner Lab Notes, Team Blog | 1 Comment

Gang Delivers at Quantum Biology Summit

Gang Ruan presented QSTORM research findings in a talk delivered today at Quantum Science Symposium 2011 in Cambridge, MA.  Gang began his talk, “Quantum Dots for Single Molecule Tracking in Live Cells,” reviewing how the Ohio State research groups he works more »

Posted in Alpert Lab Notes, Team Blog, Winter Lab Notes | 3 Comments

CMU progress update (Sep-2011)

Hi all, I am glad to announce that Yiyi has successfully passed her Ph.D. qualifier exam! Over the past few months, she had worked very hard to prepare for this exam, which usually has an attrition rate of ~20%. She more »

Posted in Team Blog, Yang Lab Notes | 1 Comment

How much quenching?

Hi Peter, We did a proof-of-concept experiment to see how much quenching effect on quantum dot (QD) we can get from gold nanoparticle close to QD. So far the most we got is ca. 70% decrease in fluorescence (see below, more »

Posted in Team Blog, Winter Lab Notes | 5 Comments

Micelles and Yellow Quantum Dots

We tested a technique this week that involved two different excitation sources.  One was a 660 nm laser and the other a 405 nm LED.  The idea being that the 405 would cause the yellow quantum dots to emit light more »

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More fish?

Peter – Would you like some more fish? We’d love to see the super-res Alexa some time. — Beth [via email]

Posted in Brainerd Lab Notes, Team Blog | 3 Comments

Quantum dots coupled with micelles

In basic terms, these are spheres of quantum dots.  This provides us a dense localized point source with many dots overlapping each other.  Therefore, if we can use blinking to localized individual dots, we are proving our method of breaking more »

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Surface modification of quantum dots for microinjection

Quantum dots directly from typical organic solvent-based synthesis are not soluble in water. We developed a novel surface modification approach based on poly(styrene-co-ethylene glycol) (PS-PEG) micelle encapsulation (Ruan and Winter, Nano Letters 2010, 2011). The features of this approach include: more »

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Structured Illumination Images

We received several fish to analyze and so I decided to perform the same method on each fish to compare and create a sort of best and worst case scenarios.  I’ll be presenting only 4 of the fish viewed simply more »

Posted in Kner Lab Notes, Team Blog | Comments Off on Structured Illumination Images