Monthly Archives: August 2011

Yang Lab Notes

The main goal of our research at CMU is to use switchable quantum dot based STORM imaging and adaptive optics to study intracellular cargo transport in neuronal cells. Our main goals in the first year include: – To establish a more »

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More fish are in the mail

Hi Peter and Andrew, I sent you 7 zebrafish larvae (5 dpf) today. There are 7 tubes and each tube has 1 fish in it. All of the fish are labelled with MF20 (the primary antibody for myosin) and Alexa more »

Posted in Brainerd Lab Notes, Team Blog | 2 Comments

How to dope nanocrystals

In response to Beth’s suggestion, I just added an entry on “doping” to the Glossary. Doping is essentially adding impurities (intentionally) to a material to achieve (large) difference in the material properties. Only in the past few years did researchers more »

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Improvement of synthesis of doped quantum dots

In the past month or so we (initially just me later graduate student Qirui Fan joined) have been synthesizing Mn-doped ZnSe quantum dots. After much trial and error, now we have obtained some product that matches the fluorescent emission wavelength more »

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Winter QSTORM lab meeting

Experimental -characterization of “good” product: absorbance, PL, TEM, quantum yield, fluorescence microscopy movie single particle imaging for brightness. Specific focus for next week: fluorescent movie. -process improvements, starting with precursor, then synthesis process. Specific focus next week: will increase reaction more »

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First QDot Tagged Fish

Hi Peter and Andrew, The fish we have sent you are our first attempt at labeling specific structures with targeted qdots. The qdots were supposed to just label the myosin thick filaments, but we don’t think that really worked in more »

Posted in Brainerd Lab Notes, Team Blog | 2 Comments

re: Photocleavable PEG

Although the estimated amount was only 0.55mg, we could request much more than that. – Gang

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Please Send More Fish

Hi Julia – Is the process for tagging the fish very involved? I ask because it would be great if, when you get the chance, you could send another couple of fish to look at. Andrew [Email]

Posted in Kner Lab Notes, Team Blog | 2 Comments

PALM Reconstruction Images

After a lot of trial and error with the program settings and modifying our system to confocal, we actually got some fairly decent pictures.  Our microscope is now capable, through coding, to take and save images on the fly.  This more »

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Photocleavable PEG

Dr. Winter, I have attached another paper with a similar photocleavable PEG (MW=5000), but the author is from Japan, so the chances might be lower. According to Gang, each test would need 100nM * 500ul quantum dots solution, and we more »

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