Monthly Archives: April 2011

Re: Questions for the Winter lab about Qdots – Gang

Hi Beth, We have had issues with clogging of injection tip. But the particles we used are two times larger than what you are using. Loss of fluorescence was observed too (but I don’t think the fluorescence loss was complete, more »

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Microinjection protocol for QDs

Hi Gang [Gang Ruan in Jessica Winter’s lab at Ohio State], Cally and Julia in my group are starting to to micoinjections of Qdots soon. Can you give us a quick sense of what has been challenging? Is it clumping/clogging more »

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Questions for the Winter lab about Qdots – Beth

Hi Gang, [Gang Ruan, post doc researcher at the Winter lab at Ohio State] Cally and Julia in my group are starting to do microinjections of Qdots soon. Can you give us a quick sense of what has been challenging? more »

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Qdots arrived – Cally

Hey guys! it’s exciting – the Qdots arrived this morning in a really big box with a tiny tiny vial inside! Cally

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Re: Microinjection with Texas Red – Beth

Hi Julia, These look great! What is your microinjection success rate? It seems like it is near 100% — you mentioned one that did not work, but it seems like all of the others did work. Or did you inject more »

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Microinjection with Texas Red – Julia

Hi Dr. Brainerd and Cally, Today I injected 6dpf fish with Texas Red. Here are the pictures. For one of the muscle fibers, I couldn’t get a zstack because I somehow managed to lose/bleach the fiber. -Julia

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Doing OK In Columbus

Just checking in guys. As you may know from my emails, I am currently battling breast cancer. I had surgery about a month ago and started chemo last week. This is expected to go through August, but believe it or more »

Posted in Team Blog, Winter Lab Notes | 4 Comments