Monthly Archives: March 2011

Re: Microinjection pictures – Beth

It is fascinating that Texas Red always labels adjacent myosepta. WHY IS THAT?? How does it travel there? And so quickly? (I assume these fish were injected and imaged right away). — B

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Microinjection pictures – Julia

Hi Dr. Brainerd and Cally, Here is a folder of muscle fiber pictures. There are two fish, and fish 1 has 2 different injection sites. Both fish were 4dpf, anesthetized, and injected with 10% 10,000 MW Texas Red conjugated with more »

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The Athens Contingent

I want to introduce Andrew who will is working on the STORM imaging in Athens and took the data that I posted last month. He’s been working on the project since last Fall when he joined my group. He’s been more »

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Re: First TEM photos – Cally

Yup, myofibrils cut in cross section (plus osmium precipitate!) and some t-tubules.  But you can see the myofilament arrangement if you stare at it long enough and look past the osmium specks. Cally

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Re: First TEM photos – Beth

Myofibrils cut in cross section, I presume? I think I can see some of the expected X-section myofilament arrangement in image 001 — Beth

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First TEM photos – Julia

Here are our first TEM photos. They aren’t great, but they tell us what we need to optimize. The really big black specks are precipitated osmium, not myosin. -Julia and Cally

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More questions about microinjection – Beth

Hi Melina — here is a list of questions. We don’t want to overwhelm you, but we are trying to be efficient by collecting all of them here. Feel free not to read them and we’ll just ask when we more »

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Phone call with Melina – Beth

Hi Melina — Thanks so much. This is really helpful. If you don’t mind, we’d still like to have a brief conversation with you tomorrow. Can be just by speakerphone to make it easy. We’ll get together a list of more »

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Re: Questions about microinjection – Melina Hale

Ah! I already see one thing that could make a big difference. We dilute our dye with filtered Hank’s solution which is sort of like a physiological extracellular solution. It includes potassium, sodium, magnesium and other chemicals that help to more »

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Questions about microinjection – Cally

Hi Melina! Here are the basic steps that we have been following for microinjection,  needle pulling, and dye concentrations: Needle Pulling: We pulled 1.0mm *0.75 mm Filling Fiber (borosilicate) capillary tubes with a delay of 3 and heat of 8. more »

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