Monthly Archives: February 2011

New QSTORM team members

Two students at Brown University have joined our QSTORM team: Cally Harper is a doctoral student and Julia Olszewski is a 3rd year undergraduate. They are working on developing the zebrafish muscle microinjection protocols and also validation of QSTORM results more »

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Re: Microinjection and TEM updates – Beth

Hi Julia and Cally — Wow! I am very impressed by your progress and your progress reports. Did you inject two fibers in the attached movies? They look pretty good — particularly for Texas red that Melina describes as “messy”. more »

Posted in Brainerd Lab Notes | Comments Off on Re: Microinjection and TEM updates – Beth

Microinjection and TEM update 2 (with picture) – Cally and Julia

This week we did the following: Microinjection: • practiced calibrating with oil droplets on the 6x system • set up a microinjection system with a 20x system • practiced with non florescent dye (phenyl red) • practiced with florescent dye more »

Posted in Brainerd Lab Notes | Comments Off on Microinjection and TEM update 2 (with picture) – Cally and Julia

Microinjection and TEM update – Julia and Cally

This week we did the following: Microinjection: • We talked to Robbert about microinjection techniques and modified our techniques (embedded fish in methyl cellulose, calibrated our picospritzer, changed our dye concentrations). • We practiced with a non florescent dye and more »

Posted in Brainerd Lab Notes | Comments Off on Microinjection and TEM update – Julia and Cally

First Microscopy Data!

We finally received our new microscope stage and tested the stability of the system. If we want to measure individual quantum dots with nanometer accuracy, we can’t have the microscope stage flying all over the place. In our test, we more »

Posted in Kner Lab Notes, Team Blog | 5 Comments